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THIS ISSUE: ASK AND THOU SHALL RECEIVE

The biggest lie perpetuated by prohibitionists and naysayers is that of the lazy stoner who is aimless and unmotivated—essentially a slacker. It might be convenient, but only if you’re willing to ignore all the innovations and advances taking place daily in the cannabis world. Luckily doing so isn’t quite so easy as they’d like, to which the articles in our feature will attest.

Do you want to keep your old mom alive forever? The Rev shows you how he does it with his ultimate seed making methods. Too old school for you? Maybe you should play mad scientist and try Bill Carrington’s futuristic tissue culture technique. Everybody knows that all the ladies love a man in a lab coat.

Maybe your grow room is your own little science lab? Prepare to experiment with Joe Pietri’s groundbreaking attempt to turn the prevailing wisdom on lighting schedules on its ass with his revolutionary 12-1 piece.

We’ve also got a “holy, how to” trilogy on butter, bhang and hashmaking for those who, like me, are hooked on the Food Network and are always looking for the next best recipe and utensils.

This issue’s MILFS hold a special place in our hearts as they are the end result of a long and arduous labor of love on the part of our friends at Green Candy Press. Excerpted from their great new book, Cannabis Indica: The Essential Guide to the World’s Finest Marijuana Strains, they represent some of the best and unique genetics from around the world. Lazy stoners indeed.
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Excerpt from:
WHAT IS TISSUE CULTURING?

words: Bill Carrington

TISSUE CULTURING consists of preserving plant genetics by taking smaller cuttings and growing them in a sugar and nutrient-rich media. Tissue culture doesn’t change the genetic material or the organism—rather, it denotes multiplying the genetic material. Unlike traditional seed or cloning you do not have to wait approx 6 months to take a new cutting, you can take a new cutting within 2-4 weeks and start the process over again. Research has shown that if you start your plant from a tissue culture and then multiply them in your lab, as opposed to multiplying them from clippings, your plants can hold the same genetic material for 20 years.

OTHER THAN YIELD, WHY DO WE WANT TO CONTROL PLANTS THROUGH CULTURING?
We all know that medical marijuana has eased the effects of many illness and chronic pain for many years. Staph have been linked to diseases stemming from molds and fungi in cloned plants. One of the best parts about tissue culturing is you can now get rid of contaminated cannabis plants. Tissue culturing will allow you to breed out viruses, molds, bacteria and yeasts that may be hibernating in your plant giving you a TRUE CLEAN medical product. We are now capable of producing therapeutic medicine that will give optimal benefits with consistency for the consumer with minimal side effects. If we generate a cannibinoid with little to no disease and minimal side effects, we will be providing contaminant-free products, which in turn will give a higher quality of medicine. This specifically will help people with a high sensitivity to molds, fungi, and yeasts. Most importantly, all plant and nutrient research has come from tissue culture research.

The hardest part about tissue culturing is contamination risks and consistency. Remember one particle of dust can carry 100s of molds, bacteria or fungus spores. As longs as you know how to keep yourself, your work area and your storage area super clean, you can tissue culture.

TISSUE CULTURING
The first step to tissue culturing is clean, clean, clean. Clean the area you will be working in by spraying and wiping down all counter surfaces. (If using a BBF Box, thoroughly wipe down inside with 25% alcohol.)

Spray and wipe down the following with your sterile napkins and place in the clean area:

  • Plate
  • Tools
  • Tool Jar (approx 2 to 4 in. tall and narrow)
  • Burner
  • Lighter
  • Sterile water jars
  • Your jars to be used for propagation

ARRANGE YOUR WORK AREA TO YOUR LIKING
If using a plastic tub for your clean area, make sure to cover area while clipping your cuttings. If you’re using a BBF Box be sure to shut the door before taking your cuttings.

Read the rest and more in SKUNK volume 7, issue 3